[No.04-0028] KAAD-Cyclopamine

SPECIFICATION

Product Name: Stemolecule KAAD-Cyclopamine

Catalog Number: 04-0028

Size: 100 µg

Alternate Name(s): N-[2-[(3′R,7’aR)-3′,6′,10,11b-tetramethyl-3-oxospiro[1,2,4,6,6a,6b,7,8, 11,11a-decahydrobenzo[a]fluorene-9,2′-3,3a,5,6,7,7a-hexahydrofuro[3, 2-b]pyridine]-4′-yl]ethyl]-6-(3-phenylpropanoylamino)hexanamide

Chemical Formula: C₄₄H₆₃N₃O₄

Molecular Weight: 697.99

CAS Number: 306387-90-6

Purity: Greater than 95% by TLC analysis

Formulation: Pale yellow solid

Solubility: For a 10 mM concentrated stock solution of KAAD-Cyclopamine, reconstitute the compound by adding 14.3 µL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37 °C for 2 to 5 minutes. For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%. This molecule is soluble in DMSO at 10 mM, ethanol at 1.4 mM, and methanol at 1.4 mM.

Storage and Stability: Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.

Quality Control: The purity of KAAD-Cyclopamine was determined by TLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of KAAD-Cyclopamine was tested on mouse embryonic stem cells.