[No.00-0055] StemAb Alkaline Phosphatase Staining Kit II
Alkaline phosphatase (AP) is expressed at high levels in pluripotent stem cells. AP dephosphorylates nucleotides, proteins, and alkaloids under alkaline conditions. After staining, undifferentiated cells appear red or purple whereas differentiated cells appear colorless.
The undifferentiated state of embryonic stem (ES) and induced pluripotent stem (iPS) cells can be characterized by a high level of alkaline phosphatase (AP) expression which, along with the expression of other surface markers, indicates undifferentiated cells with the potential to self-renew. AP is a hydrolase enzyme responsible for dephosphorylating molecules such as nucleotides, proteins, and alkaloids under alkaline conditions. When fixed ES or iPS cells are stained using the AP Staining Kit, undifferentiated cells appear red or purple, whereas differentiated cells appear colorless.
More information: reprocell.com
SPECIFICATION
Product Name: StemAb Alkaline Phosphatase Staining Kit II
Catalog Number: 00-0055
Size
50 assays
Storage and Stability
Store at 4 °C. Do not freeze. Stable for 6 months from date of receipt when stored as directed.
Quality Control
StemAb Alkaline Phosphatase Staining Kit II is functionally tested on human iPS cells and mouse ES cells to ensure product quality.
Kit Contents
- Fix Solution: 25 mL (Cat. No. 09-0042)
- AP Staining Solution A: 10 mL (Cat. No. 09-0043)
- AP Staining Solution B: 10 mL (Cat. No. 09-0065)
- AP Staining Solution C: 10 mL (Cat. No. 09-0066)
RESOURCE
Specification Sheets
Safety Data Sheets
- 00-0055 Safety Data Sheet
- 09-0042 Safety Data Sheet
- 09-0043 Safety Data Sheet
- 09-0065 Safety Data Sheet
- 09-0066 Safety Data Sheet
Protocols
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