[No.03-0019-250] Stemfactor Hepatocyte Growth Factor, Human Recombinant (250ug)
Recombinant Human Hepatocyte Growth Factor (HGF) is a paracrine cellular growth, motility and morphogenic factor. It is secreted by mesenchymal stem cells and targets and acts primarily upon epithelial and endothelial cells, but also acts on haemopoietic progenitor cells and T-cells. It has been shown to have a major role in embryonic organ development, specifically in myogenesis, in adult organ regeneration, and in wound healing.
Hepatocyte growth factor regulates cell growth, cell motility, and mophogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis and tissue regeneration.
Recombinant human HGF is an approximate 79 kDa active form of human HGF, which is a disulfide-linked heterodimer composed of α-chain (458 amino acids) and β-chain (234 amino acids) subunits. It lacks the 5 amino acid residues (F-L-P-S-S) in the 1st kringle domain; and is supplied as a glycoprotein produced by CHO cells.
More information: reprocell.com
SPECIFICATION
Product Name
Stemfactor Hepatocyte Growth Factor, Human Recombinant
Catalog Number
03-0019
Size
- 25 µg (1 µg/µL)
- 250 µg (1 µg/µL)
Purity
Greater than 99.5% by SDS-PAGE analysis.
Formulation
Dissolved in 10 mM Citrate buffer, 0.075% Tween 80, 1 M NaCl, pH 6.0. Sterilized by filtration through a 0.22 µm filter.
Storage and Stability
Store at − 70 °C.
Quality Control
Single chain form HGF <2.5% by SDS-PAGE under reducing conditions, visualized by Coomassie Brilliant Blue staining.
Sterility
Sterile filtered
Notice To Purchaser
This HGF material was produced as GMP-compatible material in a pilot scale-up run for GMP manufacturing. Custom size pharmaceutical grade GMP-compliant material is available from REPROCELL (please inquire).
Concentration
25 µL, 1 µg/µL (Cat. No. 03-0019) 250 µL, 1 µg/µL (Cat. No. 03-0019-250)
Source
Recombinant human HGF expressed in CHO cells
Endotoxin Level
Less than 0.125 EU/µg of protein as determined by the LAL method.
Biologic Activity
Biological activity was assessed based on the ability to induce proliferation of mink lung epithelial (Mv.1.Lu cells).
Manufacturer
Kringle Pharmaceutical (Japan)
RESOURCE
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