[No.00-0075] StemRNA SR Reprogramming Kit
REPROCELL’s Stemgent StemRNA-SR Reprogramming Kit is the first commercial cellular reprogramming kit combining self-replicative RNA (srRNA) and microRNA technologies to provide stem cell researchers with safe, flexible, fast and cost-effective reprogramming of both endothelial progenitor cells (EPCs) derived from human peripheral and cord blood.
The kit is comprised of one vial each of three components: OKSiM-srRNA, microRNA Reprogramming Cocktail and B18R Recombinant Protein.
More information: reprocell.com
Features include:
- Robust, validated protocol for reprogramming genetically stable EPCs derived from human peripheral or cord blood
- Virus- and DNA-free OKSiM reprogramming factors using self-replicative RNA
- Two-transfection protocol eliminates need for conditioned medium, co-transfections
TABLE 1. Efficient Establishment and Cellular srRNA Reprogramming of EPCs Derived from Human Blood Samples
| BLOOD SOURCE | PRIMARY EPC ESTABLISHMENT EFFICIENCY | PATIENT-TO-PATIENT REPROGRAMMING EFFICIENCY |
|---|---|---|
| Peripheral Blood | 18/22 = 82% | 9/13 = 70% |
| Cord Blood | 46/53 = 87% | 33/41 = 81% |
| TOTAL | 64/75 = 85% | 42/54 = 78% |
Primary EPC establishment efficiency for peripheral blood is cumulative of both fresh blood and cryopreserved mononuclear cell (MNC) samples. Cord blood efficiency data are from cryopreserved MNCs only. Each collagen-coated T-75 flask was seeded with a minimum of 5 × 107 MNCs. The unmodified StemRNA-SR kit protocol demonstrated high patient-to-patient efficiency using a single reprogramming attempt. Typical final yields are 10-20 iPS cell colonies per well in a 6-well plate format.
| Product Name | StemRNA SR Reprogramming Kit |
|---|---|
| Catalog Number | 00-0075 |
| Storage and Stability | – Store all three kit components at or below − 70 °C. |
| Quality Control | StemRNA SR Reprogramming Kit is functionally validated for successful RNA-based reprogramming of human umbilical vein endothelial cells (HUVECs). Complete reprogramming of iPS cell colonies is confirmed by expression of pluripotency markers and appropriate colony morphology. All components of the kit are sterile and have tested negative for Mycoplasma. |
| Recommended Usage | For use with associated StemRNA SR protocols for EPC derivation and generation of iPS cells. |
| Kit Contents | – OKSiM-srRNA, (Part No. 05-0038), 5 µg, 1 vial |
RESOURCES
Specification Sheets
00-0075 Product Specification Sheet
Safety Data Sheets
05-0036 Safety Data Sheet
03-0017 Safety Data Sheet
05-0038 Safety Data Sheet
Protocols
Protocol 00-0075 StemRNA-SR Reprogramming of EPCs
Product Sheets
00-0075 Product Sheet
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