A nearly transparent permeable membrane made from Type 1 Atellocolagen sized for 6-well culture plates. Amino acids and other small molecules can move freely through the permeable collagen membrane. Suitable for culture of primary epithelial cells and cell interaction studies.
Applications
- Cell culture
Features
- Microscopic observation of the cells is possible during the culture due to high membrane transparency.
- Cell culture on collagen membrane creates a natural environment that resembles in vivo systems.
Data
“Induction into layered tissue from mouse embryoid body on permeable collagen membrane and transplantation.”
(Y. Kinebuchi et al. Shinonoi General Hospital, Shinshu University, Faculty of Medicine)
Mouse embryoid body was cultivated on permeable collagen membrane in a mixed medium of keratinocyte serum-free medium and conditioned medium from 3T3 cells. These cells formed four-layered structures in vitro after 28 days culture (Fig. 1). Upon transplanting these four-layered structure, the formation of luminal walls and the expression of some epithelial cell marker, UPIII (urothelium), CK8, and CK18 (bladder) were observedin vivo(Fig. 2).