[No.04-0034] Wnt Inhibitor IWP-2

SPECIFICATION

Product Name: Stemolecule Wnt Inhibitor IWP-2

Catalog Number: 04-0034

Size: 2 mg

Alternate Name(s): N-(6-Methyl-2-benzothiazolyl)-2-[(3,4,6,7-tetrahydro-4-oxo-3-phenylthieno[3,2 d]pyrimidin-2-yl)thio]-acetamide

Chemical Formula: C₂₂H₁₈N₄O₂S₃

Molecular Weight: 466.6

CAS Number: 686770-61-6

Purity: Greater than 99 % by HPLC analysis

Formulation: White powder

Solubility: This molecule is reported to be soluble in DMSO at 5 mM.

Reconstitution: For a 5 mM concentrated stock solution of Wnt Inhibitor IWP-2, reconstitute the compound by adding 857.3 µL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37°C for 2 to 5 minutes.  For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%.

Storage and Stability: Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.

Quality Control: The purity of Wnt Inhibitor IWP-2 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of Wnt Inhibitor IWP-2 was tested on mouse embryonic stem cells.