ReproCryo DMSO Free is an optimized freezing medium for ES/iPS cells which does not employ DMSO as a functional additive. DMSO is non-desirable due to proven mutagenic and carcinogenic effects.
The medium is made from materials that meet the Japan Standards for Biological Materials Safety.
hiPSC 201B7 cell grown in ReproXF medium (Cat. No. RCHEMD007) on vitronectin were cryopreserved with ReproCryo DMSO Free and competitive media and stored at −80 °C. A vial was thawed and plated to determine viability.