[No.05-0014] Oct4 mRNA, Human (20ug)
Stemgent Oct4 mRNA encodes the Oct4 protein commonly used in cellular reprogramming systems for the generation of induced pluripotent stem (iPS) cells. This in vitro transcribed mRNA incorporates both pseudouridine and 5-methylcytidine modified nucleotides to minimize the cellular interferon response to single stranded RNA.1,2 Repeated transfection of Oct4 mRNA in combination with mRNAs encoding Klf4, Sox2, Lin-28 and c-Myc has been shown to reprogram human fibroblasts.2
Figure 1: Protein expression and localization shown by immunocytochemistry, DAPI in blue and Oct4 in red.
| Product Name | StemRNA 3rd Gen Reprogramming Kit |
|---|---|
| Catalog Number | 00-0076 |
| Storage and Stability | Store all three kit components at or below −70 °C. Kit components are stable for a minimum of 3 months from date of receipt when stored as directed. |
| Quality Control | The individual mRNAs are tested for size and integrity. The StemRNA 3rd Gen Reprogramming Kit is functionally validated for successful RNA-based reprogramming of adult fibroblasts, human umbilical vein endothelial cells (HUVECs), EPCs, and UPCs. Complete reprogramming of iPS cell colonies is confirmed by expression of pluripotency markers and appropriate colony morphology. All components of the kit are sterile and have tested negative for Mycoplasma spp. |
| Recommended Usage | For use with associated StemRNA 3rd Gen Reprogramming Kit protocols for generation of iPS cells. |
| Kit Contents | – OKSMNL NM-RNA (Part No. 05-0040), 30 µg, 1 vial |
SPECIFICATION
Product Name
Oct4 mRNA, Human
Catalog Number
05-0014
Size
20 µg
Formulation
100 ng/ul in RNase-free TE buffer, pH 7.0
Storage and Stability
Store at or below −70 °C. Stable for a minimum of 3 months from date of receipt when stored as directed. Wear gloves when handling this product. Use RNase-free tubes and barrier pipette tips. Thaw on ice. Keep on ice until the sample is removed. Avoid multiple freezing and thawing cycles (<5). Aliquot to experimental quantities.
Quality Control
Proper protein expression and localization have been confirmed by immunocytochemistry.
Related Products
- Stemgent Klf4 mRNA, Human (Cat.No.05-0015)
- Stemgent Sox2 mRNA, Human (Cat.No.05-0016)
- Stemgent c-Myc mRNA, Human (Cat.No.05-0018)
- Stemgent Lin-28 mRNA, Human (Cat.No.05-0017)
- Stemgent nGFP mRNA (Cat.No.05-0019)
- Stemgent mRNA Reprogramming Factors Set: hOKSML (Cat.No.00-0067)
- Stemfect RNA Transfection Kit (Cat.No.00-0069)
- Stemfactor Fibroblast Growth Factor-basic (Human Recombinant) (Cat.No.03-0002)
- Stemolecule Y27632 (Cat.No.04-0012)
Notice To Purchaser
This product is intended for research purposes only. It may not be used for (i) any human or veterinary use, including without limitation therapeutic and prophylactic use, (ii) any clinical use, including without limitation diagnostic and prognostic use, (iii) any use in delivery to and/or modification of cells that are intended for clinical, diagnostic or medicinal use, including without limitation, cell-based therapy, (iv) any commercial purposes, including without limitation the performance of contract research or provision of services to a third party and the manufacture of products for general sale. Any use of this product for any of the above-mentioned purposes requires a license from the Massachusetts Institute of Technology.
Recommended Usage
Stemgent Oct4 mRNA, Human should be transfected using a protocol specifically optimized for the cell type used in the experiment.
RESOURCES
Specification Sheets
Safety Data Sheets
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