[No.KKN-1494] AteloGene Local Use Quick Gelation (1 kit)
In vivo transfection kit for siRNA and microRNA
Atelocollagen, the main component of AteloGene, forms RNA-atelocollagen complexes upon mixing with synthetic siRNA or miRNA. The RNA-atelocollagen complexes are optimal for in vivo transfection, and siRNA or miRNA is effectively delivered to, and introduced into the cells.
AteloGene Local Use Quick Gelation is limited to localized administration due to its gelation feature. Gelated siRNA/atelocollagen complexes remain at the injection site, allowing siRNA and miRNA to be delivered into the cells effectively. This “quick gelation” version of AteloGene is an improved version of the prior Atelogene kit (KKN-1394). The quick gelation kit offers a faster protocol and improved results.
Simple Handling
AteloGene procedures are simple and easy — mix equal volumes of AteloGene and RNA solution, and administer the siRNA/miRNA AteloGene mixture to the mouse.
*1 Kit is sufficient for 15 injections (200 µL).
| Product Name | StemRNA 3rd Gen Reprogramming Kit |
|---|---|
| Catalog Number | 00-0076 |
| Storage and Stability | Store all three kit components at or below −70 °C. Kit components are stable for a minimum of 3 months from date of receipt when stored as directed. |
| Quality Control | The individual mRNAs are tested for size and integrity. The StemRNA 3rd Gen Reprogramming Kit is functionally validated for successful RNA-based reprogramming of adult fibroblasts, human umbilical vein endothelial cells (HUVECs), EPCs, and UPCs. Complete reprogramming of iPS cell colonies is confirmed by expression of pluripotency markers and appropriate colony morphology. All components of the kit are sterile and have tested negative for Mycoplasma spp. |
| Recommended Usage | For use with associated StemRNA 3rd Gen Reprogramming Kit protocols for generation of iPS cells. |
| Kit Contents | – OKSMNL NM-RNA (Part No. 05-0040), 30 µg, 1 vial |
SPECIFICATION
Product Name
AteloGene Local Use Quick Gelation
Catalog Number
KKN-1494
Size
1 kit
Storage and Stability
Store at 4 °C
Sterility
Sterile
Notice To Purchaser
REPROCELL is a licensed global distributor of KOKEN’s collagen-derived products everywhere, except for Japan.
Handling
1 kit allows 15 administrations
Manufacturer
Koken Co., Ltd. (Japan)
Kit contents
- AteloGene Local Use “Quick Gelation” prefilled syringe | 540µL × 3 syringes
- (Including losses during the preparation steps)
- QG buffer | 2.5mL × 1 bottle
- 2mL microtube | 4 tubes (A spare tube is included)
- 18G needle | 8 needles (2 spare needles are included
- Instruction manual | 1 leaflet
Devices and reagents required other than those in the kit:
- 1 mL disposable syringe
- 27 G needle
- siRNA/miRNA (PAGE- or HPLC-purified grade is recommended.)
- Container and water for preparation of siRNA/miRNA solution (sterilized, RNase-free)
- Cooling device (crushed ice, cold block, etc.)
- Tube rotator (that can tumble and agitate such as TAITEC RT-5, Bibby scientific SB3, etc.)
- Pipetter and tips (sterilized, RNase-free)
- High-speed refrigerated centrifuge
- Anesthetic
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